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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

human IBA1 Monoclonal antibody, PBS Only

human IBA1 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6A10

Cat no : 66827-1-PBS

Synonyms

AIF1, IBA1, G1, allograft inflammatory factor 1, AIF-1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human brain tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human gliomas using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using human IBA1 Monoclonal antibody (66827-1-Ig)

    IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66827-1-PBS targets human IBA1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen human IBA1 fusion protein Ag28236 相同性解析による交差性が予測される生物種
Full Name allograft inflammatory factor 1
Calculated molecular weight 17 kDa
GenBank accession numberBC009474
Gene symbol IBA1
Gene ID (NCBI) 199
RRIDAB_2882170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.