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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IBA1 Monoclonal antibody

IBA1 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B2A12

Cat no : 66827-2-Ig

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IBA1 Monoclonal antibody (66827-2-Ig)

    IHC staining of human tonsillitis using 66827-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IBA1 Monoclonal antibody (66827-2-Ig)

    IHC staining of human tonsillitis using 66827-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IBA1 Monoclonal antibody (66827-2-Ig)

    IHC staining of human liver cancer using 66827-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IBA1 Monoclonal antibody (66827-2-Ig)

    IHC staining of human liver cancer using 66827-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman brain tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66827-2-Ig targets IBA1 in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IBA1 fusion protein Ag28236 相同性解析による交差性が予測される生物種
Full Name allograft inflammatory factor 1
Calculated molecular weight 17 kDa
GenBank accession numberBC009474
Gene symbol IBA1
Gene ID (NCBI) 199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanIF

Antioxidants (Basel)

TNF-α Levels Are Increased in Patients with Subjective Cognitive Impairment and Are Negatively Correlated with β Amyloid-42

Authors - Sara Serafini
View Article