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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM-1/CD54 Polyclonal antibody

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30806-1-AP

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM-1 Polyclonal antibody (30806-1-AP)

    WB analysis using 30806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30806-1-AP (ICAM-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ICAM-1/CD54 Polyclonal antibody (30806-1-AP)

    IHC staining of human ovary cancer using 30806-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30806-1-AP (ICAM-1/CD54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ICAM-1/CD54 Polyclonal antibody (30806-1-AP)

    IHC staining of human ovary cancer using 30806-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30806-1-AP (ICAM-1/CD54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using ICAM-1/CD54 Polyclonal antibody (30806-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30806-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30806-1-AP (ICAM-1/CD54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using ICAM-1/CD54 Polyclonal antibody (30806-1-AP)

    IF Staining of Raji using 30806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using ICAM-1 antibody (30806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inRamos cells, L02 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30806-1-AP targets ICAM-1/CD54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).


Protocols

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 30806-1-APDownload protocol
IHC protocol for ICAM-1/CD54 antibody 30806-1-APDownload protocol
IF protocol for ICAM-1/CD54 antibody 30806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols