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  • Featured Product
  • KD/KO Validated

ICAM-1/CD54 Monoclonal antibody, PBS Only

ICAM-1/CD54 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9A8

Cat no : 60299-1-PBS

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis using 60299-1-Ig (same clone as 60299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis of Raji using 60299-1-Ig (same clone as 60299-1-PBS)

    WB result of ICAM1 antibody (60299-1-Ig, 1:50,000) with si-Control and si-ICAM1 transfected Raji cells. . This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis of HeLa using 60299-1-Ig (same clone as 60299-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis of Ramos using 60299-1-Ig (same clone as 60299-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis of Daudi using 60299-1-Ig (same clone as 60299-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis using 60299-1-Ig (same clone as 60299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    WB analysis of Raji using 60299-1-Ig (same clone as 60299-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IF Staining of human lung cancer using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IF Staining of human lung cancer using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using ICAM-1/CD54 Monoclonal antibody (60299-1-Ig)

    IF Staining of Raji using 60299-1-Ig (same clone as 60299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60299-1-PBS targets ICAM-1/CD54 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ICAM-1/CD54 fusion protein Ag8309 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).