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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM-1/CD54 Recombinant antibody, PBS Only

ICAM-1/CD54 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230342A2

Cat no : 83069-6-PBS

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Recombinant antibody (83069-6-RR)

    WB analysis using 83069-6-RR (same clone as 83069-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83069-6-RR (ICAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ICAM-1/CD54 Recombinant antibody (83069-6-RR)

    IF Staining of HeLa using 83069-6-RR (same clone as 83069-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ICAM-1/CD54 Recombinant antibody (83069-6-RR)

    IF Staining of HeLa using 83069-6-RR (same clone as 83069-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83069-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83069-6-RR

    Affinity and Kinetic Characterization of 83069-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83069-6-RR against Human ICAM-1 were performed. The affinity constant is 0.271 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83069-6-PBS targets ICAM-1/CD54 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).