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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Rat ICAM-1/CD54 Recombinant antibody, PBS Only (Capture)

ICAM-1/CD54 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240725C2

Cat no : 83696-3-PBS

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat lung using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat lung using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00678-1

    Cytometric bead array standard curve of MP00678-1

    Cytometric bead array standard curve of MP00678-1, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-3-PBS. Detection antibody: 83696-1-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00678-3

    Cytometric bead array standard curve of MP00678-3

    Cytometric bead array standard curve of MP00678-3, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-3-PBS. Detection antibody: 83696-2-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83696-3-RR

    Affinity and Kinetic Characterization of 83696-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83696-3-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP00678-1: 83696-3-PBS capture and 83696-1-PBS detection (validated in Cytometric bead array)

MP00678-3: 83696-3-PBS capture and 83696-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight60 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_012967.1
Gene symbol Icam1
Gene ID (NCBI) 25464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).