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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM-1/CD54 Recombinant antibody

ICAM-1/CD54 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240725C2

Cat no : 83696-3-RR

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    WB analysis using 83696-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    WB analysis using 83696-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat liver using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat liver using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat lung using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using ICAM-1/CD54 Recombinant antibody (83696-3-RR)

    IHC staining of rat lung using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83696-3-RR

    Affinity and Kinetic Characterization of 83696-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

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Tested Applications

Positive WB detected inrat lung tissue, rat liver tissue, rat heart tissue, rat kidney tissue, rat spleen tissue
Positive IHC detected inrat liver tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83696-3-RR targets ICAM-1/CD54 in WB, IHC, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight60 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_012967.1
Gene symbol Icam1
Gene ID (NCBI) 25464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 83696-3-RRDownload protocol
IHC protocol for ICAM-1/CD54 antibody 83696-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols