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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM2/CD102 Polyclonal antibody

ICAM2/CD102 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27322-1-AP

Synonyms

ICAM2, CD102, ICAM-2, ICAM 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM2 Polyclonal antibody (27322-1-AP)

    WB analysis using 27322-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ICAM2 Polyclonal antibody (27322-1-AP)

    WB analysis of mouse lung using 27322-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ICAM2 Polyclonal antibody (27322-1-AP)

    WB analysis of Jurkat using 27322-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHL-60 cells, mouse lung tissue, Jurkat cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27322-1-AP targets ICAM2/CD102 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM2/CD102 fusion protein Ag25952 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 2
Calculated molecular weight 31 kDa
Observed molecular weight 55-80 kDa
GenBank accession numberBC003097
Gene symbol ICAM2/CD102
Gene ID (NCBI) 3384
RRIDAB_2880843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for ICAM2/CD102 antibody 27322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols