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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM3 Polyclonal antibody

ICAM3 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 17699-1-AP

Synonyms

CD50, CDW50, ICAM 3, ICAM R, ICAM3

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 17699-1-AP

    WB analysis of Jurkat using 17699-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 17699-1-AP

    WB analysis of U-937 using 17699-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of Ramos using 17699-1-AP

    WB analysis of Ramos using 17699-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 17699-1-AP

    IP experiment of Jurkat using 17699-1-AP

    IP result of anti-ICAM3 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

  • FC experiment of Jurkat using 17699-1-AP

    FC experiment of Jurkat using 17699-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug ICAM3 antibody (17699-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

17699-1-AP targets ICAM3 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM3 fusion protein Ag11955 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 3
Calculated molecular weight 547 aa, 60 kDa
Observed molecular weight 110-160 kDa, 60 kDa
GenBank accession numberBC058903
Gene symbol ICAM3
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ICAM3 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3 antibody 17699-1-APDownload protocol
IP protocol for ICAM3 antibody 17699-1-APDownload protocol
FC protocol for ICAM3 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols