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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody

ICAM3/CD50 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241418E8

Cat no : 98097-1-RR

Synonyms

CDW50, ICAM3, ICAM R, ICAM 3, CD50

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR)

    FC experiment of human PBMCs using 98097-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR)

    FC experiment of human PBMCs using 98097-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR)

    FC experiment of human PBMCs using 98097-1-RR

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98097-1-RR

    Affinity and Kinetic Characterization of 98097-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98097-1-RR against Human ICAM3/CD50 were performed. The affinity constant is 0.366 nM.

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Tested Applications

Positive FC detected inFC receptor blocked human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98097-1-RR targets ICAM3/CD50 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 3
Calculated molecular weight 547 aa, 60 kDa
GenBank accession numberBC058903
Gene symbol ICAM3/CD50
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for ICAM3/CD50 antibody 98097-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols