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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM4 Monoclonal antibody, PBS Only

ICAM4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9B9

Cat no : 67014-1-PBS

Synonyms

CD242, ICAM 4, ICAM4, ICAM-4, LW, LW blood group protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM4 Monoclonal antibody (67014-1-Ig)

    WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human red blood cells using ICAM4 Monoclonal antibody (67014-1-Ig)

    WB analysis of human red blood cells using 67014-1-Ig (same clone as 67014-1-PBS)

    human red blood cells were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67014-1-PBS targets ICAM4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ICAM4 fusion protein Ag27628 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Calculated molecular weight 271 aa, 29 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC029364
Gene symbol ICAM4
Gene ID (NCBI) 3386
RRIDAB_2882330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.