Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12759-1-AP targets ICAM5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ICAM5 fusion protein Ag3303 相同性解析による交差性が予測される生物種 |
| Full Name | intercellular adhesion molecule 5, telencephalin |
| Calculated molecular weight | 924 aa, 97 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC026338 |
| Gene Symbol | ICAM5 |
| Gene ID (NCBI) | 7087 |
| RRID | AB_10644149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UMF0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ICAM-5, also known as telencephalin, is a type I integral membrane glycoprotein of the immunoglobulin superfamily. It is specifically expressed in the postnatal excitatory neuronal cell bodies, dendritic shafts, and dendritic filopodia of the telencephalon. ICAM-5 functions in both the immune and nervous systems. ICAM-5 strongly stimulates neurite outgrowth. It is cleaved by matrix metalloproteases upon activation of glutamate receptors or degraded through endocytosis resulting in increased spine maturation. The cleaved soluble fragment of ICAM-5 is immunosuppressive, which may be important in neuronal inflammatory diseases. (PMID: 10402269; 17682049; 25300135)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ICAM5 antibody 12759-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein. |