ICOSLG/B7-H2/CD275 Polyclonal antibody

ICOSLG/B7-H2/CD275 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14922-1-AP

Synonyms

B7H2, ICOSLG, B7 H2, B7RP-1, B7RP1


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Tested Applications

Positive WB detected inDaudi cells, JAR cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

14922-1-AP targets ICOSLG/B7-H2/CD275 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICOSLG/B7-H2/CD275 fusion protein Ag6392 相同性解析による交差性が予測される生物種
Full Name inducible T-cell co-stimulator ligand
Calculated molecular weight 33 kDa, 34 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC064637
Gene symbol ICOSLG
Gene ID (NCBI) 23308
RRIDAB_2122732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

Protocols

Product Specific Protocols
WB protocol for ICOSLG/B7-H2/CD275 antibody 14922-1-APDownload protocol
IHC protocol for ICOSLG/B7-H2/CD275 antibody 14922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.

Authors - Ha-Yeon Shin
humanIHC

Cell Mol Gastroenterol Hepatol

Sex Differences in Genomic Features of Hepatitis B-Associated Hepatocellular Carcinoma With Distinct Antitumor Immunity

Authors - Chungui Xu