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Species Reactivity

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  • Featured Product
  • KD/KO Validated

ID1 Polyclonal antibody, PBS Only

ID1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18475-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    WB analysis of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ID1 Polyclonal antibody (18475-1-AP)

    IF Staining of PC-3 using 18475-1-AP (same clone as 18475-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18475-1-AP (ID1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18475-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18475-1-PBS targets ID1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ID1 fusion protein Ag13359 相同性解析による交差性が予測される生物種
Full Name inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Calculated molecular weight 16 kDa
Observed molecular weight20 kDa
GenBank accession numberBC000613
Gene symbol ID1
Gene ID (NCBI) 3397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.