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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ID1 Monoclonal antibody, PBS Only

ID1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3G11

Cat no : 67827-1-PBS

Synonyms

bHLHb24, ID, ID1, Inhibitor of DNA binding 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ID1 Monoclonal antibody (67827-1-Ig)

    WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67827-1-Ig (ID1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ID1 Monoclonal antibody (67827-1-Ig)

    IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ID1 Monoclonal antibody (67827-1-Ig)

    IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67827-1-PBS targets ID1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ID1 fusion protein Ag13359 相同性解析による交差性が予測される生物種
Full Name inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Calculated molecular weight 16 kDa
Observed molecular weight18 kDa
GenBank accession numberBC000613
Gene symbol ID1
Gene ID (NCBI) 3397
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 18 kDa.