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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

IDE Polyclonal antibody, PBS Only

IDE Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21728-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDE Polyclonal antibody (21728-1-AP)

    WB analysis using 21728-1-AP (same clone as 21728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IDE Polyclonal antibody (21728-1-AP)

    WB analysis of HeLa using 21728-1-AP (same clone as 21728-1-PBS)

    WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IDE Polyclonal antibody (21728-1-AP)

    WB analysis of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using IDE Polyclonal antibody (21728-1-AP)

    WB analysis of A2780 using 21728-1-AP (same clone as 21728-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using IDE Polyclonal antibody (21728-1-AP)

    IP experiment of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

    IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IDE Polyclonal antibody (21728-1-AP)

    IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IDE Polyclonal antibody (21728-1-AP)

    IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IDE Polyclonal antibody (21728-1-AP)

    IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IDE Polyclonal antibody (21728-1-AP)

    IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21728-1-PBS targets IDE in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDE fusion protein Ag16392 相同性解析による交差性が予測される生物種
Full Name Abeta degrading protease
Calculated molecular weight 1019 aa, 118 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC096336
Gene symbol IDE
Gene ID (NCBI) 3416
RRIDAB_10804773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.