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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH1 Polyclonal antibody (23309-1-AP)

    WB analysis using 23309-1-AP (same clone as 23309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23309-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IDH1 Polyclonal antibody (23309-1-AP)

    IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IDH1 Polyclonal antibody (23309-1-AP)

    IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23309-1-PBS targets IDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH1 fusion protein Ag19749 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_11232028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. Its subunit structure is homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). This antibody is specific to IDH1.