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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of DU 145 using 66197-1-Ig (same clone as 66197-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of MCF-7 using 66197-1-Ig (same clone as 66197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HeLa using 66197-1-Ig (same clone as 66197-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66197-1-Ig (IDH1 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    FC experiment of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Monoclonal antibody (66197-1-Ig, Clone:2A6A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51184-1

    Cytometric bead array standard curve of MP51184-1

    Cytometric bead array standard curve of MP51184-1, IDH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66197-2-PBS. Detection antibody: 66197-1-PBS. Standard:Ag19293. Range: 3.125-200 ng/mL.

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IDH1 Monoclonal antibody, PBS Only (Detector)

IDH1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66197-1-PBS
Clone No.2A6A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66197-1-PBS targets IDH1 as part of a matched antibody pair:

MP51184-1: 66197-2-PBS capture and 66197-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH1 fusion protein Ag19293 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_2881590
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).