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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IDH1 Monoclonal antibody, PBS Only

IDH1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6A2

Cat no : 66197-1-PBS

Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • WB analysis of DU 145 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of DU 145 using 66197-1-Ig (same clone as 66197-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of MCF-7 using 66197-1-Ig (same clone as 66197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of HeLa using 66197-1-Ig (same clone as 66197-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66197-1-PBS targets IDH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH1 fusion protein Ag19293 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012846
Gene symbol IDH1
Gene ID (NCBI) 3417
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).