Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis using 15932-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15932-1-AP
WB result of IDH2 antibody (15932-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.
WB analysis of HeLa using 15932-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15932-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15932-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15932-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15932-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15932-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15932-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15932-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15932-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 15932-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15932-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15932-1-AP
IP result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.
IHC staining of human colon cancer using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15932-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 15932-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug IDH2 Polyclonal antibody (15932-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Featured Product
KD/KO Validated

IDH2 Polyclonal antibody

IDH2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 15932-1-AP

Publications(77)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

EC:1.1.1.42, ICD M, ICD-M, IDH 2, IDHM

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (77)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells, mouse heart tissue, mouse skeletal muscle tissue, mouse liver tissue, rat liver tissue, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue, HepG2 cells, K-562 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 66 publications below
IHC	See 8 publications below
IF	See 3 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

15932-1-AP targets IDH2 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8779 Product name: Recombinant human IDH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 104-452 aa of BC009244 Sequence: TQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ 相同性解析による交差性が予測される生物種
Full Name	isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight	452 aa, 51 kDa
Observed molecular weight	41-47 kDa
GenBank accession number	BC009244
Gene Symbol	IDH2
Gene ID (NCBI)	3418
RRID	AB_2264612
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48735
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 contains an N-terminal mitochondrial addressing sequence and hence is imported to the mitochondrial matrix, although localization to nuclei has also been reported. Moreover, IDH2, like ~20% of other mitochondrial enzymes, is acetylated at lysines, which inactivates the enzymatic activity.

Protocols

Product Specific Protocols
FC protocol for IDH2 antibody 15932-1-AP	Download protocol
IHC protocol for IDH2 antibody 15932-1-AP	Download protocol
IP protocol for IDH2 antibody 15932-1-AP	Download protocol
WB protocol for IDH2 antibody 15932-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
human	IHC	Immunity Age- and tissue-dependent diversity of human plasmacytoid dendritic cells uncovers a cycling subset dominant in early life and cancer. Authors - Alina Ulezko Antonova View Article
mouse	WB	Nat Metab Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics. Authors - Qi Liu View Article
human	WB	Nat Cell Biol Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism Authors - Dohun Kim View Article
human	WB	Cancer Res SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth. Authors - Xianghui Zou View Article
		Nat Commun Lactate derived from macrophages drives skin dermal fibroblasts phenotypic remodeling via MCT1-primed histone H3 lysine 23 lactylation in hypertrophic scar Authors - Yixuan Yuan View Article

Validation Data Gallery

WB analysis using 15932-1-AP

WB analysis of HEK-293 using 15932-1-AP

WB analysis of HeLa using 15932-1-AP

WB analysis of mouse heart using 15932-1-AP

WB analysis of mouse skeletal muscle using 15932-1-AP

WB analysis of mouse liver using 15932-1-AP

WB analysis of Jurkat using 15932-1-AP

WB analysis of mouse brain using 15932-1-AP

WB analysis of HEK-293 using 15932-1-AP

WB analysis of NIH/3T3 using 15932-1-AP

WB analysis of Jurkat using 15932-1-AP

WB analysis of SH-SY5Y using 15932-1-AP

WB analysis of rat liver using 15932-1-AP

IP experiment of mouse brain using 15932-1-AP

IHC staining of human colon cancer using 15932-1-AP

IHC staining of human colon cancer using 15932-1-AP

IHC staining of mouse kidney using 15932-1-AP

IHC staining of mouse kidney using 15932-1-AP

IHC staining of human colon cancer using 15932-1-AP

IHC staining of human colon cancer using 15932-1-AP

FC experiment of HeLa using 15932-1-AP

IDH2 Polyclonal antibody

IDH2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Age- and tissue-dependent diversity of human plasmacytoid dendritic cells uncovers a cycling subset dominant in early life and cancer.

Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.

Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism

SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.

Lactate derived from macrophages drives skin dermal fibroblasts phenotypic remodeling via MCT1-primed histone H3 lysine 23 lactylation in hypertrophic scar

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