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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis using 23254-1-AP (same clone as 23254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of Jurkat using 23254-1-AP (same clone as 23254-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of mouse brain using 23254-1-AP (same clone as 23254-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of DU 145 using 23254-1-AP (same clone as 23254-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of HEK-293 using 23254-1-AP (same clone as 23254-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using IDH2 Polyclonal antibody (23254-1-AP)

    WB analysis of mouse liver using 23254-1-AP (same clone as 23254-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IDH2 Polyclonal antibody (23254-1-AP)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IDH2 Polyclonal antibody (23254-1-AP)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH2 Polyclonal antibody (23254-1-AP)

    IF Staining of HepG2 using 23254-1-AP (same clone as 23254-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23254-1-AP (IDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

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IDH2 Polyclonal antibody, PBS Only

IDH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23254-1-PBS targets IDH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH2 fusion protein Ag19748 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 41-47 kDa
GenBank accession numberBC009244
Gene Symbol IDH2
Gene ID (NCBI) 3418
RRIDAB_2879241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48735
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved. This antibody is specific to IDH2.