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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IDH2 Recombinant antibody, PBS Only

IDH2 Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1

Cat no : 82117-1-PBS

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH2 Recombinant antibody (82117-1-RR)

    WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IDH2 Recombinant antibody (82117-1-RR)

    WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using IDH2 Recombinant antibody (82117-1-RR)

    IP experiment of mouse brain using 82117-1-RR (same clone as 82117-1-PBS)

    IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IDH2 Recombinant antibody (82117-1-RR)

    IHC staining of human colon cancer using 82117-1-RR (same clone as 82117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82117-1-PBS targets IDH2 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDH2 fusion protein Ag8779 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight43 kDa
GenBank accession numberBC009244
Gene symbol IDH2
Gene ID (NCBI) 3418
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.