Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
68199-1-PBS targets IDH3B in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IDH3B fusion protein Ag8693 相同性解析による交差性が予測される生物種 |
| Full Name | isocitrate dehydrogenase 3 (NAD+) beta |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 39-42 kDa |
| GenBank accession number | BC001960 |
| Gene Symbol | IDH3B |
| Gene ID (NCBI) | 3420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43837 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).