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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using IDI1 Polyclonal antibody (11166-2-AP)

    WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using IDI1 Polyclonal antibody (11166-2-AP)

    WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using IDI1 Polyclonal antibody (11166-2-AP)

    WB analysis of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using IDI1 Polyclonal antibody (11166-2-AP)

    WB analysis of human liver using 11166-2-AP (same clone as 11166-2-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using IDI1 Polyclonal antibody (11166-2-AP)

    IP experiment of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

    IP result of anti-IDI1 (IP:11166-2-AP, 4ug; Detection:11166-2-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IDI1 Polyclonal antibody (11166-2-AP)

    IHC staining of human colon cancer using 11166-2-AP (same clone as 11166-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IDI1 Polyclonal antibody (11166-2-AP)

    IHC staining of human liver using 11166-2-AP (same clone as 11166-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IDI1 Polyclonal antibody (11166-2-AP)

    IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IDI1 Polyclonal antibody (11166-2-AP)

    IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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IDI1 Polyclonal antibody, PBS Only

IDI1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11166-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11166-2-PBS targets IDI1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDI1 fusion protein Ag1636 相同性解析による交差性が予測される生物種
Full Name isopentenyl-diphosphate delta isomerase 1
Calculated molecular weight 31 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC019227
Gene Symbol IDI1
Gene ID (NCBI) 3422
RRIDAB_2233532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13907
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IDI1(Isopentenyl-diphosphate Delta-isomerase 1) is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). It belongs to the IPP isomerase type 1 family. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 26 kDa and 32 kDa.