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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDO1 Monoclonal antibody (66528-1-Ig)

    WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using IDO1 Monoclonal antibody (66528-1-Ig)

    WB analysis of HSC-T6 using 66528-1-Ig (same clone as 66528-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using IDO1 Monoclonal antibody (66528-1-Ig)

    WB analysis of 4T1 using 66528-1-Ig (same clone as 66528-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IDO1 Monoclonal antibody (66528-1-Ig)

    IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using IDO1 Monoclonal antibody (66528-1-Ig)

    IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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IDO1 Monoclonal antibody, PBS Only

IDO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66528-1-PBS
Clone No.3G2G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66528-1-PBS targets IDO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDO1 fusion protein Ag3953 相同性解析による交差性が予測される生物種
Full Name indoleamine 2,3-dioxygenase 1
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
RRIDAB_2881891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14902
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).