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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IDO1 Recombinant antibody (84063-1-RR)

    WB analysis of HeLa using 84063-1-RR (same clone as 84063-1-PBS)

    IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 84063-1-RR (IDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84063-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00982-3

    Sandwich ELISA standard curve of MP00982-3

    Sandwich ELISA standard curve of MP00982-3, Human IDO1 Recombinant Matched Antibody Pair - PBS only. 84063-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3953. 84063-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP00982-3

    Sample test of MP00982-3

    A549 cells were cultured unstimulated or stimulated with 300 μg/mL IFN-gamma for 5 days. The mean IDO1 concentration was determined to be 0.30 ng/mL in unstimulated A549 supernatant, 1.19 ng/mL in IFN-gamma stimulated A549 supernatant.

  • Sample test of MP00982-3

    Sample test of MP00982-3

    Serum of fifteen individual healthy human donors was measured. The human IDO1 concentration of detected samples was determined to be 8.21 ng/mL with a range of ND - 41.58 ng/mL. (ND = Non-detectable)

  • Flow cytometry (FC) experiment of HeLa cells using IDO1 Recombinant antibody (84063-1-RR)

    FC experiment of HeLa using 84063-1-RR (same clone as 84063-1-PBS)

    1x10^6 IFN-gamma treated HeLa cells were intracellularly stained with 0.25 ug Ido1 Recombinant Antibody (84063-1-RR, Clone:241110C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84063-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00982-2

    Cytometric bead array standard curve of MP00982-2

    Cytometric bead array standard curve of MP00982-2, IDO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84063-1-PBS. Detection antibody: 84063-3-PBS. Standard: Ag3953. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00982-3

    Cytometric bead array standard curve of MP00982-3

    Cytometric bead array standard curve of MP00982-3, IDO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84063-1-PBS. Detection antibody: 84063-4-PBS. Standard: Ag3953. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 84063-1-RR

    Affinity and Kinetic Characterization of 84063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84063-1-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

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IDO1 Recombinant antibody, PBS Only (Capture)

IDO1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84063-1-PBS
Clone No.241110C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84063-1-PBS targets IDO1 as part of a matched antibody pair:

MP00982-2: 84063-1-PBS capture and 84063-3-PBS detection (validated in Cytometric bead array)

MP00982-3: 84063-1-PBS capture and 84063-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDO1 fusion protein Ag3953 相同性解析による交差性が予測される生物種
Full Name indoleamine 2,3-dioxygenase 1
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14902
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).