• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IDO1 Recombinant antibody

IDO1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241110F2

Cat no : 84063-3-RR

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IDO1 Recombinant antibody (84063-3-RR)

    WB analysis using 84063-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84063-3-RR (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDO1 Recombinant antibody (84063-3-RR)

    IHC staining of mouse brain using 84063-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84063-3-RR

    Affinity and Kinetic Characterization of 84063-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84063-3-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inrat lung tissue, human plancta tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84063-3-RR is IDO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDO1 fusion protein Ag3953 相同性解析による交差性が予測される生物種
Full Name indoleamine 2,3-dioxygenase 1
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight42 kDa
GenBank accession numberBC027882
Gene symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Protocols

Product Specific Protocols
WB protocol for IDO1 antibody 84063-3-RRDownload protocol
IHC protocol for IDO1 antibody 84063-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols