Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat lung tissue, human plancta tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84063-3-RR targets IDO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IDO1 fusion protein Ag3953 相同性解析による交差性が予測される生物種 |
| Full Name | indoleamine 2,3-dioxygenase 1 |
| Calculated molecular weight | 403 aa, 45 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC027882 |
| Gene Symbol | IDO1 |
| Gene ID (NCBI) | 3620 |
| RRID | AB_3671633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14902 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IDO1 antibody 84063-3-RR | Download protocol |
| IHC protocol for IDO1 antibody 84063-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |