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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using IER2 Polyclonal antibody (23849-1-AP)

    IHC staining of human liver using 23849-1-AP (same clone as 23849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IER2 Polyclonal antibody (23849-1-AP)

    IHC staining of human liver using 23849-1-AP (same clone as 23849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IER2 Polyclonal antibody (23849-1-AP)

    IHC staining of human kidney using 23849-1-AP (same clone as 23849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23849-1-AP (IER2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 23849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IER2 Polyclonal antibody (23849-1-AP)

    IHC staining of human kidney using 23849-1-AP (same clone as 23849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23849-1-AP (IER2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 23849-1-PBS in a different storage buffer formulation.

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IER2 Polyclonal antibody, PBS Only

IER2 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 23849-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23849-1-PBS targets IER2 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IER2 fusion protein Ag20869 相同性解析による交差性が予測される生物種
Full Name immediate early response 2
Calculated molecular weight 223 aa, 24 kDa
GenBank accession numberBC072432
Gene Symbol IER2
Gene ID (NCBI) 9592
RRIDAB_2879339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9BTL4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ier2 (also called ETR101, Pip92, 3CH92 and CHX1, hereafter referred to as Ier2, regardless of species) is an immediate early gene (PMID: 2678945) that was originally found to be upregulated in fibroblasts upon their activation (PMID: 3841511). Immediate early genes are a group of genes that are rapidly induced in quiescent cells by proliferation- and migration-inducing stimuli. Ier2 may have a role in tumor cell motility and metastasis (PMID: 22120713).