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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    WB analysis of mouse brain using 16041-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    WB analysis of mouse brain using 16041-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    WB analysis of human brain using 16041-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    WB analysis of mouse testis using 16041-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    IHC staining of human brain using 16041-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IFFO1 Polyclonal antibody (16041-1-AP)

    IHC staining of human brain using 16041-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IFFO1 Polyclonal antibody (16041-1-AP)

    IF Staining of HeLa using 16041-1-AP

    Immunofluorescent analysis of Hela cells, using IFFO1 antibody 16041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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IFFO1 Polyclonal antibody

IFFO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16041-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tumor antigen HOM-TES-103, Non-homologous end joining factor IFFO1, NHEJ factor IFFO1, IFFO, HOM TES 103

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16041-1-AP targets IFFO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFFO1 fusion protein Ag8915 相同性解析による交差性が予測される生物種
Full Name intermediate filament family orphan 1
Calculated molecular weight 62 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC004384
Gene Symbol IFFO1
Gene ID (NCBI) 25900
RRIDAB_2121676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0D2I5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFFO1, also named as IFFO and Tumor antigen HOM-TES-103, is a member of the intermediate filament family which consists of primordial components of the cytoskeleton and nuclear envelope that are mainly distributed in the nucleus, followed by the cytoskeleton and cytoplasm. IFFO1 in the nucleus was identified to combine with both XRCC4 and the nuclear lamina protein lamin A/C to be involved in nonhomologous DNA end joining (NHEJ). The expression of IFFO1 is associated with tumor progression. IFFO1 could be used as a potential marker to differentiate malignant ascites cell-free tumor DNA (cftDNA) of ovarian cancer from benign controls.

Protocols

Product Specific Protocols
WB protocol for IFFO1 antibody 16041-1-APDownload protocol
IHC protocol for IFFO1 antibody 16041-1-APDownload protocol
IF protocol for IFFO1 antibody 16041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Lett

METTL3-mediated N6-methyladenosine modification and HDAC5/YY1 promote IFFO1 downregulation in tumor development and chemo-resistance

Authors - Ye Zhang
View Article