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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI16 Polyclonal antibody

IFI16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29280-1-AP

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFI16 Polyclonal antibody (29280-1-AP)

    WB analysis using 29280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29280-1-AP (IFI16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using IFI16 Polyclonal antibody (29280-1-AP)

    IHC staining of human renal cell carcinoma using 29280-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using IFI16 Polyclonal antibody (29280-1-AP)

    IHC staining of human renal cell carcinoma using 29280-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inDaudi cells, Jurkat cells, MOLT-4 cells, Ramos cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29280-1-AP targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFI16 fusion protein Ag30297 相同性解析による交差性が予測される生物種
Full Name interferon, gamma-inducible protein 16
Calculated molecular weight 729 aa, 82 kDa
Observed molecular weight85-95 kDa
GenBank accession numberBC017059
Gene symbol IFI16
Gene ID (NCBI) 3428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

Protocols

Product Specific Protocols
WB protocol for IFI16 antibody 29280-1-APDownload protocol
IHC protocol for IFI16 antibody 29280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols