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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFI35 Recombinant antibody

IFI35 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240063C6

Cat no : 83160-2-RR

Synonyms

interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IFI35 Recombinant antibody (83160-2-RR)

    IF Staining of HeLa using 83160-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using IFI35 Recombinant antibody (83160-2-RR)

    FC experiment of A549 using 83160-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HeLa cells using IFI35 Recombinant antibody (83160-2-RR)

    FC experiment of HeLa using 83160-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83160-2-RR

    Affinity and Kinetic Characterization of 83160-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83160-2-RR targets IFI35 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFI35 fusion protein Ag34653 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein 35
Calculated molecular weight 31 kDa
GenBank accession numberBC001356
Gene symbol IFI35
Gene ID (NCBI) 3430
RRIDAB_3670853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

Protocols

Product Specific Protocols
IF protocol for IFI35 antibody 83160-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols