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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFIH1/MDA5 Monoclonal antibody, PBS Only

IFIH1/MDA5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8B11

Cat no : 66770-1-PBS

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    IHC staining of human kidney using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IFIH1/MDA5 Monoclonal antibody (66770-1-Ig)

    IHC staining of human kidney using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66770-1-PBS targets IFIH1/MDA5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFIH1/MDA5 fusion protein Ag17448 相同性解析による交差性が予測される生物種
Full Name interferon induced with helicase C domain 1
Calculated molecular weight 1025 aa, 117 kDa
Observed molecular weight 117-125 kDa
GenBank accession numberBC111750
Gene symbol IFIH1
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).