IFIT1 Polyclonal antibody
IFIT1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29897-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | IFN alpha treated HeLa cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29897-1-AP targets IFIT1 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFIT1 fusion protein Ag31980 相同性解析による交差性が予測される生物種 |
Full Name | interferon-induced protein with tetratricopeptide repeats 1 |
Calculated molecular weight | 478 aa, 55 kDa |
Observed molecular weight | 55 KDa |
GenBank accession number | BC007091 |
Gene symbol | IFIT1 |
Gene ID (NCBI) | 3434 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.
Protocols
Product Specific Protocols | |
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WB protocol for IFIT1 antibody 29897-1-AP | Download protocol |
IHC protocol for IFIT1 antibody 29897-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |