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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFIT1 Recombinant antibody

IFIT1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240132G11

Cat no : 83423-1-RR

Synonyms

IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFIT1 Recombinant antibody (83423-1-RR)

    WB analysis of HeLa using 83423-1-RR

    IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using IFIT1 Recombinant antibody (83423-1-RR)

    WB analysis of SGC-7901 using 83423-1-RR

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFIT1 Recombinant antibody (83423-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFIT1 Recombinant antibody (83423-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using IFIT1 Recombinant antibody (83423-1-RR)

    FC experiment of HeLa using 83423-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83423-1-RR

    Affinity and Kinetic Characterization of 83423-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

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Tested Applications

Positive WB detected inIFN alpha treated HeLa cells, SGC-7901 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83423-1-RR is IFIT1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFIT1 fusion protein Ag19691 相同性解析による交差性が予測される生物種
Full Name interferon-induced protein with tetratricopeptide repeats 1
Calculated molecular weight 478 aa, 55 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC007091
Gene symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

Protocols

Product Specific Protocols
WB protocol for IFIT1 antibody 83423-1-RRDownload protocol
IHC protocol for IFIT1 antibody 83423-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols