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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFIT3 Monoclonal antibody, PBS Only

IFIT3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C8B10

Cat no : 67208-1-PBS

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFIT3 Monoclonal antibody (67208-1-Ig)

    WB analysis using 67208-1-Ig (same clone as 67208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67208-1-Ig (IFIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IFIT3 Monoclonal antibody (67208-1-Ig)

    IHC staining of human spleen using 67208-1-Ig (same clone as 67208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IFIT3 Monoclonal antibody (67208-1-Ig)

    IHC staining of human spleen using 67208-1-Ig (same clone as 67208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67208-1-Ig (IFIT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IFIT3 Monoclonal antibody (67208-1-Ig)

    IF Staining of HepG2 using 67208-1-Ig (same clone as 67208-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFIT3 antibody (67208-1-Ig, Clone: 2C8B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67208-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67208-1-PBS targets IFIT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFIT3 fusion protein Ag7518 相同性解析による交差性が予測される生物種
Full Name IFIT3
Calculated molecular weight 56 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC004977
Gene symbol IFIT3
Gene ID (NCBI) 3437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.