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  • Featured Product
  • KD/KO Validated

IFITM1-Specific Monoclonal antibody, PBS Only

IFITM1-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5B5E2

Cat no : 60074-1-PBS

Synonyms

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13

Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    WB analysis of TF-1 using 60074-1-Ig (same clone as 60074-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    WB analysis using 60074-1-Ig (same clone as 60074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    WB analysis of K-562 using 60074-1-Ig (same clone as 60074-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    WB analysis of HepG2 using 60074-1-Ig (same clone as 60074-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    WB analysis of human testis using 60074-1-Ig (same clone as 60074-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    IP experiment of HepG2 using 60074-1-Ig (same clone as 60074-1-PBS)

    IP result of anti-IFITM1-Specific (IP:60074-1-Ig, 4ug; Detection:60074-1-Ig 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    IHC staining of human lymphoma using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using IFITM1-Specific Monoclonal antibody (60074-1-Ig)

    IF Staining of K-562 using 60074-1-Ig (same clone as 60074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IFITM1-Specific antibody (60074-1-Ig, Clone: 5B5E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60074-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60074-1-PBS targets IFITM1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFITM1-Specific fusion protein Ag2320 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2233405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.