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  • Featured Product
  • KD/KO Validated

IFITM2/3 Monoclonal antibody, PBS Only

IFITM2/3 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E8D12

Cat no : 66081-1-PBS

Synonyms

1 8U, IFITM2/3, IFITM3, IP15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFITM2/3 Monoclonal antibody (66081-1-Ig)

    WB analysis using 66081-1-Ig (same clone as 66081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IFITM2/3 Monoclonal antibody (66081-1-Ig)

    IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IFITM2/3 Monoclonal antibody (66081-1-Ig)

    IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using IFITM2/3 Monoclonal antibody (66081-1-Ig)

    IF Staining of U2OS using 66081-1-Ig (same clone as 66081-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66081-1-PBS targets IFITM2/3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFITM2/3 fusion protein Ag17863 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 3 (1-8U)
Observed molecular weight 15-20 kDa
GenBank accession numberBC070243
Gene symbol IFITM3
Gene ID (NCBI) 10410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.