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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFITM2 Recombinant antibody (83455-5-RR)

    WB analysis using 83455-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83455-5-RR (IFITM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IFITM2 Recombinant antibody (83455-5-RR)

    IF Staining of HepG2 using 83455-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IFITM2 Recombinant antibody (83455-5-RR)

    IF Staining of HepG2 using 83455-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using IFITM2 Recombinant antibody (83455-5-RR)

    FC experiment of MCF-7 using 83455-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM2 Recombinant antibody (83455-5-RR, Clone:240318B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83455-5-RR

    Affinity and Kinetic Characterization of 83455-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83455-5-RR against Human IFITM2 were performed. The affinity constant is below 1 pM.

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IFITM2 Recombinant antibody

IFITM2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83455-5-RR
Clone No.240318B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 240318B6, 18D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, MCF-7 cells, A549 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83455-5-RR targets IFITM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFITM2 fusion protein Ag3451 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009696
Gene Symbol IFITM2
Gene ID (NCBI) 10581
RRIDAB_3671091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for IFITM2 antibody 83455-5-RRDownload protocol
IF protocol for IFITM2 antibody 83455-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols