IFITM2 Recombinant antibody
IFITM2 Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240318B6
Cat no : 83455-5-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, SGC-7901 cells, MCF-7 cells, A549 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 83455-5-RR is IFITM2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IFITM2 fusion protein Ag3451 相同性解析による交差性が予測される生物種 |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Calculated molecular weight | 132 aa, 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC009696 |
| Gene symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2 antibody 83455-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
