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Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using IFN Alpha Monoclonal antibody (66162-1-Ig)

    WB analysis of Transfected HEK-293 using 66162-1-Ig (same clone as 66162-1-PBS)

    WB result of Interferon alpha antibody (66162-1-Ig; 1:300; room temperature for 1.5 hours) with negative control and Interferon alpha overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 66162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using IFN Alpha Monoclonal antibody (66162-1-Ig)

    WB analysis of Recombinant protein using 66162-1-Ig (same clone as 66162-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66162-1-Ig (Interferon alpha antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66162-1-PBS in a different storage buffer formulation.

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IFN Alpha Monoclonal antibody, PBS Only

IFN Alpha Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66162-1-PBS
Clone No.4A2A11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, IFN-alpha, LeIF D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66162-1-PBS targets IFN Alpha in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFN Alpha fusion protein Ag12533 相同性解析による交差性が予測される生物種
Full Name IFNA1
Calculated molecular weight 189 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC074928
Gene Symbol IFN alpha1
Gene ID (NCBI) 3439
RRIDAB_2881558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01562
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.