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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFN-gamma Polyclonal antibody

IFN-gamma Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29788-1-AP

Synonyms

IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFN gamma Polyclonal antibody (29788-1-AP)

    WB analysis using 29788-1-AP

    PMA, Ionomycin and Brefeldin A treated CTLL-2 cells were subjected to SDS PAGE followed by western blot with 29788-1-AP (IFN gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated CTLL-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29788-1-AP targets IFN-gamma in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name interferon gamma
Calculated molecular weight17 kDa
Observed molecular weight15-18 kDa
GenBank accession numberBC119063
Gene symbol IFN gamma
Gene ID (NCBI) 15978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

Protocols

Product Specific Protocols
WB protocol for IFN-gamma antibody 29788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols