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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFN gamma Polyclonal antibody (28722-1-AP)

    WB analysis using 28722-1-AP

    Non-treated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 28722-1-AP (IFNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IFN-gamma Polyclonal antibody

IFN-gamma Polyclonal Antibody for WB, ELISA
Cat No. 28722-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFNG, IFNγ, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28722-1-AP targets IFN-gamma in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFN-gamma fusion protein Ag30371 相同性解析による交差性が予測される生物種
Full Name IFN gamma
Calculated molecular weight 166 aa, 19 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC070256
Gene Symbol IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_3086082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Protocols

Product Specific Protocols
WB protocol for IFN-gamma antibody 28722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols