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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFNGR1 Polyclonal antibody (31951-1-AP)

    WB analysis using 31951-1-AP

    EL-4 cells were subjected to SDS PAGE followed by western blot with 31951-1-AP (IFN-gamma R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of EL-4 cells using IFNGR1 Polyclonal antibody (31951-1-AP)

    IP experiment of EL-4 using 31951-1-AP

    IP result of anti-IFNGR1 (IP:31951-1-AP, 4ug; Detection:31951-1-AP 1:600) with EL-4 cells lysate 2080 ug.

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IFNGR1 Polyclonal antibody

IFNGR1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 31951-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Synonyms

Ifngr, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN-gamma R1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inEL-4 cells
Positive IP detected inEL-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31951-1-AP targets IFNGR1 in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interferon gamma receptor 1
Calculated molecular weight52kDa
Observed molecular weight90 kDa
GenBank accession numberNM_010511.3
Gene Symbol Ifngr1
Gene ID (NCBI) 15979
RRIDAB_3670157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP15261
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Protocols

Product Specific Protocols
WB protocol for IFNGR1 antibody 31951-1-APDownload protocol
IP protocol for IFNGR1 antibody 31951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols