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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFRD1 Polyclonal antibody (12939-1-AP)

    WB analysis of HeLa using 12939-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12939-1-AP (IFRD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using IFRD1 Polyclonal antibody (12939-1-AP)

    WB analysis of human skeletal muscle using 12939-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12939-1-AP (IFRD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFRD1 Polyclonal antibody (12939-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12939-1-AP (IFRD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFRD1 Polyclonal antibody (12939-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12939-1-AP (IFRD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IFRD1 Polyclonal antibody

IFRD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12939-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Nerve growth factor-inducible protein PC4, Interferon-related developmental regulator 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human skeletal muscle tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12939-1-AP targets IFRD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFRD1 fusion protein Ag3988 相同性解析による交差性が予測される生物種
Full Name interferon-related developmental regulator 1
Calculated molecular weight 451 aa, 50 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC001272
Gene Symbol IFRD1
Gene ID (NCBI) 3475
RRIDAB_10597099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFRD1 antibody 12939-1-APDownload protocol
IHC protocol for IFRD1 antibody 12939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Translation of 5' leaders is pervasive in genes resistant to eIF2 repression.

Authors - Dmitry E Andreev
View Article
humanWB

RNA Biol

Divergent effects of translation termination factor eRF3A and nonsense-mediated mRNA decay factor UPF1 on the expression of uORF carrying mRNAs and ribosome protein genes.

Authors - Affaf Aliouat
View Article