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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using IFRD2 Polyclonal antibody (26952-1-AP)

    WB analysis of HL-60 using 26952-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IFRD2 Polyclonal antibody (26952-1-AP)

    WB analysis of HeLa using 26952-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFRD2 Polyclonal antibody (26952-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IFRD2 Polyclonal antibody (26952-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IFRD2 Polyclonal antibody

IFRD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26952-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Interferon-related developmental regulator 2, IFNRP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26952-1-AP targets IFRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitybovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFRD2 fusion protein Ag25057 相同性解析による交差性が予測される生物種
Full Name interferon-related developmental regulator 2
Calculated molecular weight 48 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007265
Gene Symbol IFRD2
Gene ID (NCBI) 7866
RRIDAB_2880700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFRD2, or interferon-related developmental regulator 2, is a protein that has been identified as a novel factor capable of translationally inactivating ribosomes. It plays a role in regulating gene expression by directly binding to 80S ribosomes, which precludes the binding of mRNA and P-site tRNA, thereby inhibiting translation

Protocols

Product Specific Protocols
WB protocol for IFRD2 antibody 26952-1-APDownload protocol
IHC protocol for IFRD2 antibody 26952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB,IF

J Muscle Res Cell Motil

IFRD2, a target of miR-2400, regulates myogenic differentiation of bovine skeletal muscle satellite cells via decreased phosphorylation of ERK1/2 proteins

Authors - Zhian Gong
View Article