IFT122 Polyclonal antibody
IFT122 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, canine
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19304-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human brain tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
19304-1-AP targets IFT122 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFT122 fusion protein Ag6860 相同性解析による交差性が予測される生物種 |
| Full Name | intraflagellar transport 122 homolog (Chlamydomonas) |
| Calculated molecular weight | 142 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC004238 |
| Gene symbol | IFT122 |
| Gene ID (NCBI) | 55764 |
| RRID | AB_2714184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT122 antibody 19304-1-AP | Download protocol |
| IHC protocol for IFT122 antibody 19304-1-AP | Download protocol |
| IF protocol for IFT122 antibody 19304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Elife A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia. | ||
Hum Mol Genet Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. |
