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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    WB analysis of mouse testis using 19304-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19304-1-AP (IFT122 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human testis using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human testis using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human stomach using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human stomach using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human brain using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IFT122 Polyclonal antibody (19304-1-AP)

    IHC staining of human brain using 19304-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of MDCK using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of hTERT-RPE1 using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT122 Polyclonal antibody (19304-1-AP)

    IF Staining of hTERT-RPE1 using 19304-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

IFT122 Polyclonal antibody

IFT122 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19304-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WDR10p, WDR10, WD repeat-containing protein 140, WD repeat-containing protein 10, SPG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19304-1-AP targets IFT122 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6860

Product name: Recombinant human IFT122 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-359 aa of BC004238

Sequence: MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGLWSPEQKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREERNDILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSGVKEREWQME

相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 122 homolog (Chlamydomonas)
Calculated molecular weight 142 kDa
Observed molecular weight140 kDa
GenBank accession numberBC004238
Gene Symbol IFT122
Gene ID (NCBI) 55764
RRIDAB_2714184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBG6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT122 antibody 19304-1-APDownload protocol
IHC protocol for IFT122 antibody 19304-1-APDownload protocol
IF protocol for IFT122 antibody 19304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua
View Article
mouseWB,IF

Elife

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Authors - Tooba Quidwai
View Article
humanWB

Hum Mol Genet

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Authors - Mariko Takahara
View Article