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- KD/KO Validated
IFT52 Polyclonal antibody
IFT52 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine and More (1)
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17534-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells |
| Positive IP detected in | mouse testis tissue, HeLa cells |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
17534-1-AP targets IFT52 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFT52 fusion protein Ag11586 相同性解析による交差性が予測される生物種 |
| Full Name | intraflagellar transport 52 homolog (Chlamydomonas) |
| Calculated molecular weight | 437 aa, 50 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC039831 |
| Gene symbol | IFT52 |
| Gene ID (NCBI) | 51098 |
| RRID | AB_2280005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT52 antibody 17534-1-AP | Download protocol |
| IF protocol for IFT52 antibody 17534-1-AP | Download protocol |
| IP protocol for IFT52 antibody 17534-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. | ||
Am J Hum Genet Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice. | ||
Am J Hum Genet Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
Proc Natl Acad Sci U S A FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Charlotte (Verified Customer) (07-22-2024) | I tested the antibody following the classic iF protocol: PFA 4% 10 min cold methanol 5 min 1% BSA in PBST 1/200 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS However, the result is very doty which makes it hard to interpret and to distinguish IFT52 real signal (signal should be similar to the ARL13B one or as dots along a line if not showing a continuous line). It could be due to the protocol that needs to be adjusted for this antibody or maybe the antibody works better in other cell lines
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