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KD/KO Validated

IFT74 Polyclonal antibody

Name: IFT74 Polyclonal antibody
Brand: Proteintech
SKU: 27334-1-AP

IFT74 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27334-1-AP

Synonyms

CCDC2, CMG 1, CMG1, IFT74

Validation Data Gallery

Filter:

AllWB

WB analysis of NIH/3T3 using 27334-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 27334-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 27334-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 27334-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 27334-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Cat No. 27334-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

The immunogen of 27334-1-AP is IFT74 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IFT74 fusion protein Ag26385 相同性解析による交差性が予測される生物種
Full Name	intraflagellar transport 74 homolog (Chlamydomonas)
Calculated molecular weight	600 aa, 69 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC107742
Gene symbol	IFT74
Gene ID (NCBI)	80173
RRID	AB_2880847
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT74 antibody 27334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Genet Med Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome. Authors - Minna Luo KD Validated View Article
human	WB	Pulm Circ Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia Authors - Saejeong Park View Article
human,mouse	IF	Hum Reprod Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility Authors - Qi Li View Article
human	WB,IP	Hum Mol Genet Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations Authors - Mahmoud R Fassad View Article
human	WB	EMBO J The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport Authors - Niels Boegholm KO Validated View Article
mouse	WB,CoIP	EMBO Mol Med IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect Authors - Jing Dai View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT74 Polyclonal antibody

IFT74 Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 27334-1-AP

WB analysis of Jurkat using 27334-1-AP

WB analysis of HEK-293 using 27334-1-AP

WB analysis of Raji using 27334-1-AP

WB analysis of mouse thymus using 27334-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.

Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia

Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility

Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations

The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport

IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect