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IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13967-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

TTC10, TG737, Intraflagellar transport protein 88 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cell, MDCK cells, mouse thymus tissue
Positive IP detected inknockout cells and WT cells, HEK-293 cells
Positive IHC detected inmouse heart tissue, human pancreas tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13967-1-AP targets IFT88 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT88 fusion protein Ag4980 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
RRIDAB_2121979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-APDownload protocol
IHC protocol for IFT88 antibody 13967-1-APDownload protocol
IF protocol for IFT88 antibody 13967-1-APDownload protocol
IP protocol for IFT88 antibody 13967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.

Authors - Firestone Ari J AJ
mouseWB

Nature

Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O
chickenIF

Science

Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.

Authors - Raman M Das
mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
humanIF

Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin
mouseIF

Nat Genet

Complex interactions between genes controlling trafficking in primary cilia.

Authors - Ocbina Polloneal Jymmiel R PJ
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