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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using IFT88 Monoclonal antibody (60227-1-Ig)

    WB analysis of NCCIT using 60227-1-Ig (same clone as 60227-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using IFT88 Monoclonal antibody (60227-1-Ig)

    IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

    IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IFT88 Monoclonal antibody (60227-1-Ig)

    IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

IFT88 Monoclonal antibody, PBS Only

IFT88 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA
Cat No. 60227-1-PBS
Clone No.4A4G5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Synonyms

hTg737, IFT88, TG737, TPR repeat protein 10, TTC10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60227-1-PBS targets IFT88 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFT88 fusion protein Ag4980 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight 94 kDa
Observed molecular weight 88-95 kDa
GenBank accession numberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
RRIDAB_11042449
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13099
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.