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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT88 Recombinant antibody

IFT88 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230074F8

Cat no : 82886-1-RR

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074F8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT88 Recombinant antibody (82886-1-RR)

    WB analysis using 82886-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-1-RR (IFT88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IFT88 Recombinant antibody (82886-1-RR)

    IHC staining of mouse heart using 82886-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82886-1-RR (IFT88 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82886-1-RR

    Affinity and Kinetic Characterization of 82886-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-1-RR against Human IFT88 were performed. The affinity constant is 49.8 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, human testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82886-1-RR targets IFT88 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT88 fusion protein Ag4980 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight 94 kDa
Observed molecular weight 88-95 kDa
GenBank accession numberBC030776
Gene symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 82886-1-RRDownload protocol
IHC protocol for IFT88 antibody 82886-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols