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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT88 Recombinant antibody

IFT88 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230074E12

Cat no : 82886-2-RR

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT88 Recombinant antibody (82886-2-RR)

    WB analysis using 82886-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT88 Recombinant antibody (82886-2-RR)

    IF Staining of hTERT-RPE1 using 82886-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Flow cytometry (FC) experiment of HEK-293 cells using IFT88 Recombinant antibody (82886-2-RR)

    FC experiment of HEK-293 using 82886-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82886-2-RR

    Affinity and Kinetic Characterization of 82886-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, PC-3 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82886-2-RR is IFT88 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFT88 fusion protein Ag4980 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight 94 kDa
Observed molecular weight 88-95 kDa
GenBank accession numberBC030776
Gene symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 82886-2-RRDownload protocol
IF protocol for IFT88 antibody 82886-2-RRDownload protocol
FC protocol for IFT88 antibody 82886-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols